Journal: Immunity

Article Title: Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock

doi: 10.1016/j.immuni.2018.06.011

Figure Lengend Snippet: (A–B) Kaplan-Meier survival plot (left) and body temperature plot (right) over time (hpc) in LPS-challenged WT (n=19), Ripk3−/− (n=15), Casp8+/−Ripk3−/− (n=8), Casp8−/−Ripk3−/− (n=26) and Casp11−/− (n=11) mice (A) or WT (n=6), Ifnar1−/− (n=8) and Irf3−/− (n=5) mice (B). (C) Kaplan-Meier survival plot for WT (n=6), littermate Casp8+/−Ripk3−/− (n=6) and Casp8−/−Ripk3−/− (n=7) as well as Casp11−/− mice (n=6) given a poly(I:C) prime (4 mg/kg) followed 6 h later with low dose LPS challenge (5 mg/kg). (D) Kaplan-Meier survival plot for WT (n=19), littermate Casp8+/−Ripk3−/− (n=20) and Casp8−/−Ripk3−/− (n=15) mice over time, h post infection (hpi) with E. coli (ATCC 25922). Groups here included balanced numbers of male and female animals. Statistical comparisons (to WT) was by Log-Rank (Mantel-Cox) test (* p<0.05; ** p<0.01). Please also see Figure S1.

Article Snippet: These data reveal a crucial requirement for Casp8 in TLR4-dependent endotoxemia beyond the recognized role of the type I IFN-Casp11-Gsdmd axis ( Kayagaki et al., 2015 ; Kayagaki et al., 2011 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 caption a8 Casp8 drives endotoxic shock (A–B) Kaplan-Meier survival plot (left) and body temperature plot (right) over time (hpc) in LPS-challenged WT (n=19), Ripk3 −/− (n=15), Casp8 +/− Ripk3 −/− (n=8), Casp8 −/− Ripk3 −/− (n=26) and Casp11 −/− (n=11) mice (A) or WT (n=6), Ifnar1 −/− (n=8) and Irf3 −/− (n=5) mice (B). (C) Kaplan-Meier survival plot for WT (n=6), littermate Casp8 +/− Ripk3 −/− (n=6) and Casp8 −/− Ripk3 −/− (n=7) as well as Casp11 −/− mice (n=6) given a poly(I:C) prime (4 mg/kg) followed 6 h later with low dose LPS challenge (5 mg/kg). (D) Kaplan-Meier survival plot for WT (n=19), littermate Casp8 +/− Ripk3 −/− (n=20) and Casp8 −/− Ripk3 −/− (n=15) mice over time, h post infection (hpi) with E. coli (ATCC 25922).

Techniques: Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Genotypes of isogenic and UPEC strains a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Ciprofloxacin MICs for E. coli strains in urine at different pH values a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: MICs of ciprofloxacin for isogenic E. coli strains in MH broth at different pH values a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Mean fold changes in MICs of ciprofloxacin in MH broth and urine at different values of pH and anaerobiosis a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques: